专利名称：Methods and compositions for generation
of multiple copies of nucleic acid sequencesand methods of detection thereof
发明人：Nurith Kurn申请号：US10857160申请日：20040528
公开号：US20050064456A1公开日：20050324
摘要：The present invention provides novel isothermal methods of generatingmultiple copies of, detecting and/or quantifying nucleic acid sequences of interest basedon limited primer extension or attachment of oligonucleotide pairs using compositeRNA/DNA primers. Methods for generating multiple copies of and/ordetecting and/orquantifying nucleic acid sequences, wherein products of primer extension or attachmentof oligonucleotide pairs comprising a cleavable portion are generated, and whereincleavage of the products results in dissociation of cleaved products from targetpolynucleotides, are provided. The invention further provides compositions, kits andsystems for practicing these methods.
申请人：Nurith Kurn
地址：Palo Alto CA US
国籍：US
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