Detection of Antibodies

专利名称：Detection of Antibodies发明人：David Beeson申请号：US12097399申请日：20061214

公开号：US20090029388A1公开日：20090129

摘要：The present invention relates to a method for detecting antibodies against atarget antigen in a sample which comprises contacting the sample with labelled targetantigen, subjecting the sample to immunoprecipitation to precipitate antibodies in thesample and detecting the presence of antibodies against the target antigen in thesample by means of the presence of labelled target antigen in the immunoprecipitate,wherein the labelled target antigen is a fusion protein comprising the target antigen anda fluorescent protein label and the presence of labelled target antigen in the

immunoprecipitate is detected by means of the fluorescence of the fluorescent label.The method is particularly suitable for use where the target antigen is an autoantigenand can also be used to identify autoantigens implicated in a particular autoimmunedisorder by screening serum samples from patients with a clinical phenotype indicative orsuggestive of an autoimmune disorder and suitable controls. The target protein may befrom the cys-loop acetyl choline receptor ion channel gene superfamily, the voltage-gated calcium, sodium or potassium ion channel gene superfamily, the glutamate

receptor gene family, a receptor tyrosine kinase, or other membrane associated channelssuch as aquaporin gene family.

申请人：David Beeson

地址：Oxford GB

国籍：GB

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